Both larvae carry an attP docking site integrated into the genome via piggyBac-mediated germline transformation using a cyan fluorescent marker (see article by Labbé et al., doi:10.1371/journal.pntd.0000788).
The top larva also carries a 3xP3-DsRed2 marker — leading to expression of DsRed2 red fluorescent protein and hence prominent red fluorescence in the eyes and optic nerve — integrated into the attP site via PhiC31-mediated site-specific integration. Development of gene transfer technology for Aedes albopictus is a key step in the study and control of this invasive species using novel molecular techniques and genetic control strategies.
Image credit: Geneviève Labbé/Oxitec Ltd/PloS Neglected Tropical Diseases, August 2010. This image was published under the Creative Commons Attribution License (CCAL).