Three-dimensional confocal image reconstruction of a murine intestinal villus tip infected with L. monocytogenes expressing InlAm, InlB, and green fluorescent protein (GFP). Six hours after orogastric gavage, the terminal ileum was imaged with phalloidin in red to visualise actin, TO-PRO-3 in blue to see the nuclei, and GFP in green to see invading bacteria. Several replicating cytosolic bacteria surrounded by actin clouds or tails have invaded the villus tip epithelial cells. InlB accelerates invasion of the villus epithelium (see Pentecost et al., doi:10.1371/journal.ppat.1000900).
Image credit: Mickey Pentecost and Manuel R Amieva, Stanford University School of Medicine
This image was published under the Creative Commons Attribution License (CCAL).